In histological practice, the procedure involves preparing a tissue sample from a biopsy or surgical specimen and staining it with the Heppar1 antibody. The stained tissue is then examined under a microscope. A positive result is indicated by brownish cytoplasmic staining in hepatocytes. This method is particularly beneficial when used alongside other markers such as alpha-fetoprotein (AFP) and Glypican-3 to improve diagnostic accuracy.