The detection of HBV in histological samples typically involves several techniques:
Hematoxylin and Eosin (H&E) Staining: This is a standard staining method used to observe general tissue morphology and identify inflammation and cell damage. Immunohistochemistry (IHC): This technique uses antibodies specific to HBV antigens, such as HBsAg (Hepatitis B surface antigen) and HBcAg (Hepatitis B core antigen), to detect the presence of the virus in liver tissue. In Situ Hybridization (ISH): This method can detect HBV DNA within hepatocytes, providing direct evidence of viral infection.
