FITC is commonly conjugated to antibodies in immunofluorescence assays. These FITC-labeled antibodies can bind to specific antigens in tissue sections. When the tissue is exposed to light of the appropriate wavelength, the FITC fluoresces, allowing the location of the antigen to be visualized under a fluorescence microscope. This technique is widely used for diagnosing diseases, studying cell signaling pathways, and understanding tissue architecture.
