Several histological techniques are employed in brain mapping:
1. Immunohistochemistry: This technique uses antibodies to detect specific proteins in brain tissues, helping to identify different cell types and their connections. 2. In situ hybridization: It allows for the localization of specific nucleic acid sequences within the tissue, providing insights into gene expression patterns. 3. Electron microscopy: This provides ultra-high resolution images of brain tissue, revealing the detailed structure of cells and their synaptic connections. 4. Confocal microscopy: Used to obtain high-resolution, three-dimensional images of brain tissues, enabling the study of complex networks of neurons. 5. Nissl staining: A classic technique that highlights cell bodies, allowing for the visualization of neuronal density and distribution.
