fluorescein isothiocyanate (fitc)

How to Optimize the Use of FITC in Histological Studies?

To maximize the effectiveness of FITC in histological applications, consider the following tips:
Minimize Exposure to Light: Protect FITC-labeled samples from prolonged light exposure to reduce photobleaching.
Use Appropriate Filters: Employ suitable excitation and emission filters to enhance the specificity and sensitivity of detection.
Optimize Antibody Concentrations: Use optimal antibody concentrations to balance signal intensity and background noise.

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