DNA quality is typically assessed using several techniques:
Spectrophotometry: Measures DNA concentration and purity based on absorbance ratios at 260/280 nm and 260/230 nm. Gel electrophoresis: Assesses DNA integrity by visualizing DNA fragments on an agarose gel; high-quality DNA appears as high-molecular-weight bands. Fluorometry: Uses fluorescent dyes to specifically quantify double-stranded DNA, providing more accurate measurements than spectrophotometry. qPCR: Evaluates DNA quality by amplifying specific gene targets; less degraded DNA will amplify more efficiently.
