dna extraction

How is DNA Quality and Quantity Assessed?

After extraction, the quality and quantity of DNA need to be assessed to ensure it is suitable for downstream applications. This can be done using several methods:
Spectrophotometry: Measuring the absorbance at 260 nm (A260) to determine DNA concentration and using the A260/A280 ratio to assess purity.
Fluorometry: Using fluorescent dyes that specifically bind to DNA to measure concentration.
Gel Electrophoresis: Running the DNA on an agarose gel to assess the integrity and size distribution of the extracted DNA.

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