Several methods are employed to measure volume fraction in histology:
Point Counting: A stereological technique where a grid of points is overlaid on tissue sections, and the fraction of points hitting the component of interest is counted. Image Analysis: Digital imaging software is used to analyze stained tissue sections. The software calculates the area occupied by different components and converts this to volume fraction. Cavalieri Principle: This method involves slicing the tissue into uniform sections and calculating the area of the component of interest in each section. The total volume is then estimated.
