The process of cryosectioning involves several steps:
1. Sample Preparation: The tissue sample is first embedded in a medium, usually OCT compound (Optimal Cutting Temperature compound), which provides support and stability during sectioning. 2. Freezing: The embedded tissue is rapidly frozen using a cryostat, which maintains the tissue at a very low temperature (typically around -20°C to -30°C). 3. Sectioning: The frozen tissue is then cut into thin sections, usually between 5 to 20 micrometers thick, using the cryostat. 4. Mounting: The thin tissue sections are mounted on glass slides for subsequent staining and microscopic analysis.
