Despite its advantages, cryosectioning also has some limitations:
- Tissue Integrity: Rapid freezing can sometimes cause the formation of ice crystals, which can damage the tissue and affect the quality of the sections. - Handling and Storage: Frozen sections are delicate and require careful handling. They also need to be stored at very low temperatures to prevent thawing and degradation. - Limited Long-Term Storage: Unlike paraffin-embedded sections, cryosections are not ideal for long-term storage as they can degrade over time.
