The process of counterstaining typically involves the following steps:
Fixation: The tissue sample is fixed to preserve its structure and composition. Primary Staining: The sample is stained with a primary stain that targets specific cellular components. Rinsing: Excess primary stain is rinsed off to prevent non-specific staining. Counterstaining: The sample is then stained with a counterstain, which provides contrast to the primary stain. Dehydration: The sample is dehydrated through a series of alcohol baths to prepare it for mounting. Mounting: The sample is mounted on a slide with a coverslip for microscopic examination.
