After obtaining a tissue sample via EBUS, the specimen is immediately placed in a fixative solution to preserve its cellular structure. The sample is then processed in the histology lab, where it undergoes embedding, sectioning, and staining. Common staining techniques include Hematoxylin and Eosin (H&E) and immunohistochemical stains, which help to identify specific cell types and markers. The prepared slides are examined under a microscope by a pathologist to determine the presence of disease.
