Histological studies of Col4a4 involve various techniques to visualize and analyze its distribution and function within tissues. Immunohistochemistry (IHC) is commonly used to detect Col4a4 in tissue sections using specific antibodies. This technique allows researchers to observe the localization and expression levels of Col4a4 in different tissues and under various pathological conditions. Electron Microscopy provides ultra-structural details of the basement membrane, revealing alterations in its structure due to Col4a4 mutations. Additionally, genetic sequencing can identify specific mutations in the Col4a4 gene, aiding in the diagnosis of related diseases.
