Chemical balancing involves maintaining the correct concentrations of reagents used in tissue processing and staining. This includes:
Fixatives: The concentration and type of fixative must be balanced to preserve tissue structure without causing excessive cross-linking or shrinkage. Stains: The proportion of dyes and their interactions with tissue components need to be optimized to achieve clear and specific staining results. pH Levels: The pH of staining solutions can affect the affinity of dyes for tissue components, necessitating careful adjustment.
