To improve analytical accuracy, histologists use various techniques:
Routine staining: Techniques like Hematoxylin and Eosin (H&E) staining provide clear differentiation of tissue components. Special stains: Techniques such as PAS, Masson's Trichrome, and Silver stains highlight specific tissue components or pathogens. Immunohistochemistry: Uses antibodies to detect specific proteins, aiding in identifying disease markers. Molecular techniques: Techniques like in situ hybridization (ISH) and PCR help in analyzing genetic material within tissues.
