Performing PCR on tissue samples involves several steps:
Sample Preparation: Tissue samples are often fixed and embedded in paraffin before being sectioned for analysis. It is crucial to ensure that the DNA is adequately preserved during this process. DNA Extraction: DNA is extracted from the tissue sections using chemical or enzymatic methods. The quality and purity of the extracted DNA are essential for successful PCR. Amplification: The extracted DNA is subjected to PCR, using specific primers to target the desired DNA sequence.
