CD117 is typically detected using immunohistochemistry (IHC), a technique that employs antibodies to detect specific antigens in tissue sections. The primary antibody binds to CD117, and a secondary antibody conjugated with a detectable marker (such as an enzyme or fluorescent dye) binds to the primary antibody. This allows for the visualization of CD117 expression in tissue samples. Other methods, such as flow cytometry and Western blotting, can also be used to detect CD117 expression.
