Histological study of bone involves several techniques:
Decalcification: Bone samples are treated with acid solutions to remove minerals, making them soft enough for sectioning. H&E Staining: Hematoxylin and eosin staining highlights the cellular components and matrix of the bone. Special Stains: Techniques like Masson's trichrome and Von Kossa staining are used to visualize specific components such as collagen fibers and mineral deposits. Microscopy: Light and electron microscopy provide detailed images of the bone structure and cellular components.
