In histological studies, axonal damage is identified through various staining techniques and imaging methods. Commonly used methods include:
Silver Staining: This technique is used to visualize axons and detect any degenerative changes. Immunohistochemistry: Specific antibodies are used to identify proteins associated with axonal damage such as amyloid precursor protein (APP). Electron Microscopy: Provides high-resolution images of axonal ultrastructure, allowing for the observation of detailed morphological changes. Fluorescent Labeling: Markers such as neurofilament proteins can be tagged with fluorescent dyes to study axonal integrity.
