In histology, apoptosis resistance can be studied using various techniques:
- Immunohistochemistry: This technique can be used to detect the expression of proteins involved in apoptosis, such as Bcl-2, p53, and caspases, in tissue samples.
- TUNEL Assay: The TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) assay is used to detect DNA fragmentation, a hallmark of apoptosis, in tissue sections.
- Electron Microscopy: This can provide detailed images of the ultrastructural changes associated with apoptosis, such as chromatin condensation and membrane blebbing.
- Western Blotting: This technique can be used to analyze the expression of apoptosis-related proteins in tissue extracts.
