Several issues can arise during the decalcification process:
Over-Decalcification: Prolonged exposure to decalcifying agents can lead to tissue damage and loss of cellular detail. Under-Decalcification: Insufficient decalcification can result in hard tissue remnants, making sectioning difficult and damaging microtome blades. pH Control: Maintaining the appropriate pH is crucial, especially when using acid decalcifiers, to prevent tissue deterioration.
