Detecting antigen heterogeneity requires advanced histological techniques. Some of the commonly used methods include:
1. Immunohistochemistry (IHC): This technique uses antibodies to detect specific antigens in tissue sections. Variations in staining intensity and pattern can indicate heterogeneity. 2. Flow Cytometry: This method can analyze the expression of multiple antigens in individual cells, providing a quantitative measure of heterogeneity. 3. Single-Cell RNA Sequencing: This advanced technique allows for the analysis of gene expression at the single-cell level, revealing heterogeneity at a molecular level. 4. Multiplex Immunofluorescence: This technique enables the simultaneous detection of multiple antigens in a single tissue section, providing a comprehensive view of antigen diversity.
