After the tissue sample is collected, it undergoes several steps before microscopic examination:
Fixation: The tissue is preserved using chemicals like formalin to prevent degradation. Embedding: The fixed tissue is embedded in a solid medium, usually paraffin wax, to provide support for sectioning. Sectioning: Thin slices of the tissue are cut using a microtome. Staining: The tissue sections are stained with dyes such as Hematoxylin and Eosin (H&E) to highlight cellular structures. Microscopic Examination: A pathologist examines the stained tissue sections under a microscope.
