Preparation of a suspension involves several steps: 1. Tissue Disaggregation: The tissue sample is enzymatically or mechanically disaggregated to release individual cells. 2. Filtration: The cell suspension is filtered to remove clumps and debris. 3. Centrifugation: The cells are often concentrated by centrifugation and resuspended in a suitable medium. 4. Medium Selection: The choice of medium depends on the downstream application and may include buffers, nutrients, or specific reagents to maintain cell viability.
