3β-HSD can be detected through various histological techniques such as immunohistochemistry (IHC) and in situ hybridization (ISH). In IHC, antibodies specific to 3β-HSD are used to bind to the enzyme, which can then be visualized using chromogenic or fluorescent labels. ISH involves using labeled complementary RNA or DNA probes to detect 3β-HSD mRNA within tissue sections.
