Recent advancements in mass spectrometry have significantly enhanced its application in histological studies. Improved mass spectrometry techniques have led to better spatial resolution, increased sensitivity, and more accurate quantitative analyses. Techniques such as Matrix-Assisted Laser Desorption/Ionization (MALDI), Secondary Ion Mass Spectrometry (SIMS), and Liquid Chromatography-Mass Spectrometry (LC-MS) have been optimized to provide more detailed and specific data.