The process of scRNA-seq involves several steps. First, cells are isolated from a tissue sample using methods such as fluorescence-activated cell sorting (FACS) or microfluidics. Each cell is then lysed to release its RNA, which is reverse transcribed into complementary DNA (cDNA). The cDNA is subsequently amplified and sequenced. Advanced bioinformatics tools are used to analyze the resulting sequences, enabling the quantification of gene expression levels in individual cells.
