Sample preparation is a critical step in histological imaging. Proper preparation ensures that tissues are adequately preserved, stained, and sectioned to reveal the desired structures. Fixation is usually the first step, which involves preserving tissues using chemicals like formaldehyde. This is followed by embedding the tissue in a medium such as paraffin or resin, and then sectioning it into thin slices using a microtome. Staining techniques, like hematoxylin and eosin (H&E), are then applied to highlight different cellular components.
