What are the Common Imaging Techniques in Histology?
Several imaging techniques are commonly used in histology, each with its own advantages and limitations:
Light Microscopy: The most traditional method, using visible light to illuminate the sample. Common variations include brightfield, phase contrast, and differential interference contrast microscopy. Fluorescence Microscopy: Uses fluorescent dyes to label specific cellular components, allowing for high-contrast imaging of targeted structures. Confocal Microscopy: Provides enhanced resolution and depth by using laser scanning technology to produce optical sections of thick specimens. Electron Microscopy: Offers exceptional resolution by using electron beams instead of light. It includes transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Multiphoton Microscopy: Similar to confocal but uses longer wavelength light, reducing photodamage and allowing deeper tissue penetration.
