An autoclave uses steam sterilization, which involves heating water to produce steam. The steam is then pressurized to achieve higher temperatures necessary for sterilization. The combination of heat and pressure effectively kills all forms of microbial life, including spores. The typical cycle consists of:
Heating: The autoclave heats up to the required temperature. Sterilization: The contents are exposed to high-pressure steam for a specific period. Cooling: The autoclave gradually depressurizes and cools down.
