The cryostat operates by maintaining a chamber at sub-zero temperatures, typically between -20°C to -30°C. Tissue samples are embedded in a medium like Optimal Cutting Temperature (OCT) compound, which provides support during sectioning. A microtome within the cryostat then slices the tissue into thin sections, usually around 5-10 micrometers thick. These sections are then mounted on slides for further staining and examination.
