Cryostat sectioning is crucial for several reasons:
Rapid Diagnosis: In clinical settings, cryostats enable quick preparation of tissue sections, allowing for immediate examination and diagnosis. Preservation of Antigens: The freezing process preserves the native state of antigens, making cryostat sections ideal for immunohistochemical staining. Minimal Chemical Alteration: Unlike paraffin-embedded sections, cryostat sections are not exposed to harsh chemicals, preserving the tissue's biochemical integrity.