PCR inhibitors can impact the amplification process in several ways:
- Enzyme Inhibition: Inhibitors can alter the structure or function of DNA polymerases, reducing their ability to synthesize DNA. - Template Binding: Some inhibitors can bind to the DNA template, preventing the polymerase from accessing the DNA for amplification. - Chelation: Certain substances can chelate magnesium ions, an essential cofactor for polymerase activity, thus reducing the efficiency of the PCR.
