In histology, antibody-based agents work on the principle of antigen-antibody binding. This highly specific interaction occurs between the antibody and its corresponding antigen. The process typically involves two types of antibodies: primary and secondary. The primary antibody directly binds to the target antigen, while the secondary antibody, which is often conjugated with a detectable marker such as an enzyme or fluorophore, binds to the primary antibody, enhancing the signal and enabling visualization.
