To avoid fixative dilution, adhere to the following guidelines: 1. Correct Volumes: Use an appropriate volume of fixative relative to the tissue size. Typically, a volume ratio of 10:1 (fixative to tissue) is recommended. 2. Fresh Preparation: Always prepare fresh fixative solutions or ensure that stock solutions are not expired. 3. Proper Storage: Store fixatives in tightly sealed containers to avoid evaporation and contamination. 4. Quality Control: Regularly check the concentration of your fixative solutions using appropriate analytical methods.
