Several strategies can be employed to mitigate reduced signal intensity:
Optimizing Fixation: Ensuring proper tissue fixation can help preserve cellular structures and maintain signal intensity. Standardizing Protocols: Following standardized staining and imaging protocols can reduce variability and improve signal consistency. Using High-Quality Reagents: Utilizing high-quality antibodies and reagents can enhance signal intensity and specificity. Minimizing Photobleaching: Reducing exposure to light during fluorescence microscopy can help preserve fluorophore signal intensity.
