Irreversible changes can be identified through various histological techniques:
- Staining techniques: Different stains can help identify cellular and tissue damage. For example, H&E (Hematoxylin and Eosin) staining can show cell death and tissue architecture disruption. - Microscopy: Using light and electron microscopes, pathologists can observe the ultrastructural changes in cells and tissues that indicate irreversible damage. - Immunohistochemistry: This technique uses antibodies to detect specific antigens in tissues, helping to identify specific types of cell death or damage.
