How is Histological Analysis Conducted in Aquaculture?
Histological analysis in aquaculture typically involves several steps: 1. Sample Collection: Tissues are collected from the organism, often from organs such as the liver, gills, intestines, and muscles. 2. Fixation: Tissues are preserved using fixatives like formaldehyde to maintain their structure and prevent decay. 3. Embedding: Fixed tissues are embedded in paraffin wax to provide support for slicing. 4. Sectioning: Thin sections of the tissue are cut using a microtome. 5. Staining: Sections are stained with dyes such as hematoxylin and eosin to highlight different cellular components. 6. Microscopy: Stained sections are examined under a microscope to identify histological features and abnormalities.