How are Trophozoites and Cysts Identified in Histological Sections?
Identification of trophozoites and cysts in histological sections involves the use of specific stains and observation of their morphological features. Common staining methods include Hematoxylin and Eosin (H&E) stain, Periodic Acid-Schiff (PAS) stain, and special stains like Giemsa or Trichrome stain. Trophozoites are generally identified by their active appearance, presence of organelles, and motility structures such as flagella or pseudopodia. Cysts, on the other hand, are recognized by their thick wall, size, and the presence of multiple nuclei or internal reorganization structures.
