Preparing specimens for light microscopy typically involves several steps:
Fixation: Preserves the tissue and prevents degradation. Embedding: Involves placing the tissue in a solid medium, usually paraffin, to provide support for sectioning. Sectioning: Thin slices of tissue are cut using a microtome. Staining: Applied to highlight different structures within the tissue, using dyes such as Hematoxylin and Eosin (H&E).
