Histologists use various techniques to study signaling pathways, including:
Immunohistochemistry (IHC): Detects specific proteins within tissue sections using antibodies. In Situ Hybridization (ISH): Localizes specific nucleic acid sequences within tissue sections. Western Blotting: Analyzes protein expression and post-translational modifications. Fluorescence Microscopy: Visualizes signaling molecules tagged with fluorescent markers. Flow Cytometry: Measures cell surface and intracellular molecules, aiding in the analysis of cell signaling.
