How are Samples Prepared for Cytogenetic Analysis?
Sample preparation is crucial for accurate cytogenetic analysis. The steps generally include: 1. Collection: Samples can be collected from various tissues, including blood, bone marrow, amniotic fluid, and solid tissues. 2. Cell Culture: Cells are cultured to ensure that they divide and provide enough material for analysis. 3. Harvesting: Cells are harvested at the right stage of cell division (usually metaphase) when chromosomes are most visible. 4. Staining and Mounting: Chromosomes are stained using dyes like Giemsa or fluorescent probes and then mounted on slides for microscopy.
