Rimmed vacuoles are identified using histological staining techniques. The most common techniques include Hematoxylin and Eosin (H&E) staining, which highlights the basophilic rim, and Gomori Trichrome staining, which can accentuate the vacuoles. Electron microscopy may also be used to provide a more detailed view, revealing the presence of filamentous inclusions and other ultrastructural abnormalities.
