STED (Stimulated Emission Depletion) microscopy is a form of super-resolution microscopy that surpasses the diffraction limit of light, allowing for imaging at the nanometer scale. This technique is particularly useful in fields like histology where high-resolution images of tissues and cells are crucial. STED achieves super-resolution by selectively depleting the fluorescence in the periphery of the excitation spot, leaving a smaller region that can be imaged with enhanced clarity.
