How are Proteins, DNA, and RNA Studied in Histology?
Histological techniques to study proteins, DNA, and RNA include:
Immunohistochemistry (IHC): This technique uses antibodies to detect specific proteins in tissue sections. It is widely used for diagnosing diseases and understanding protein localization. Fluorescence In Situ Hybridization (FISH): FISH uses fluorescent probes to bind to specific DNA sequences, allowing for the visualization of genetic abnormalities. In Situ Hybridization (ISH): ISH involves using labeled RNA or DNA probes to detect specific RNA sequences in tissue sections, aiding in the study of gene expression. Western Blotting: Though not a traditional histological technique, it is often used in conjunction with histology to identify and quantify proteins extracted from tissues.