Detection of pro inflammatory cytokines in tissues involves several methods:
Immunohistochemistry: This technique uses antibodies specific to the cytokines to visualize their presence in tissue sections. In Situ Hybridization: This method detects the mRNA of cytokines, indicating their production at the transcriptional level. ELISA (Enzyme-Linked Immunosorbent Assay): Though not a histological technique, ELISA can quantify cytokines in tissue extracts.
