How are Paraffin Sections Prepared for Microscopy?
After embedding, sections are cut using a microtome. The typical thickness of paraffin sections is around 4-6 micrometers. The sections are then mounted on microscope slides and deparaffinized using solvents like xylene, followed by rehydration through a graded series of alcohols. Finally, the sections are stained with appropriate dyes to highlight various cellular components.
