The use of paraffin in histology involves several steps: 1. Fixation: Tissues are first fixed to preserve cellular structures. 2. Dehydration: Water is removed from tissues using a series of alcohol baths. 3. Clearing: Tissues are then cleared using a solvent like xylene to remove alcohol and make the tissue receptive to paraffin. 4. Infiltration: Tissues are infiltrated with molten paraffin at temperatures typically around 60°C. 5. Embedding: Infiltrated tissues are embedded in paraffin blocks, which are allowed to solidify.
