How are Nucleic Acid Sequences Identified in Tissue Samples?
Several molecular techniques are employed to identify nucleic acid sequences in tissue samples:
1. Polymerase Chain Reaction (PCR): This technique amplifies specific DNA sequences, making it possible to analyze even minute quantities of DNA extracted from tissues. 2. In Situ Hybridization (ISH): ISH uses labeled complementary DNA or RNA probes to localize specific nucleic acid sequences within tissue sections. 3. Next-Generation Sequencing (NGS): This high-throughput technology allows for the comprehensive analysis of nucleic acid sequences, enabling the identification of gene expression patterns and mutations within tissues.
