Identifying non viable cells can be achieved through various histological techniques. Common methods include:
1. Staining: Vital stains such as Trypan Blue and Propidium Iodide are used to distinguish non viable cells. Non viable cells uptake these dyes, appearing blue or red under a microscope, while live cells exclude them. 2. Morphological Changes: Non viable cells often exhibit characteristic morphological changes such as cell shrinkage, membrane blebbing, and nuclear condensation. 3. Markers of Apoptosis: Detection of specific markers like Annexin V, which binds to phosphatidylserine exposed on the outer leaflet of the plasma membrane, is indicative of apoptotic cells.
